Methods of Injection

1. Syringe Injection The sample can be injected on the front of the system, which will pass directly onto the column. The samples need to be filtered before injection. We also offer syringe filters from Whatman, so the sample can be filtered directly into the injection port on the system. This works well with samples that are thick or have a lot of particulate.
2.  Liquid Reservoir Injection This allows for small to larger volumes of liquid to be injected. It works similar to syringe injection, except the sample is injected by pressurizing the liquid reservoir. It also provides a way to stop the injection and wash the entire sample onto the column.
3.  Direct Solid/liquid Reservoir Injection This method can be used for samples that are difficult to inject on the column, like thick liquids with particulate. The advantage of this is no sample filtering before injection. This injection system goes directly into the injection port on the system or can be placed on top of the column. A filter (RTSI-50) is place on top of the end fitting which has a 10-20 µm glass frit inside. The sample is added and pressurized onto the column.
4.  Solid Reservoir Injection This method provides an effective way to inject samples that have been pre-absorbed to silica. This injection system goes in line with the column and allows the removal of the air in the injection reservoir before the sample is passed onto the column. The column can be pre-condition before solvent is passed though the injection reservoir.
5.  Direct Solid/liquid Reservoir Injection This method is good for samples of low solubility that can be pre-absorbed and added to reservoir column as above. This injection system goes directly on top of the column and the pre-absorbed sample is added. This can also be used to add a liquid sample onto silica similar to a conventional column.
6.  EZ-Pass with Direct Solid/liquid Reservoir Injection This method allows the pre-absorbed sample to be prepared in the injection column. All steps take place in the injection column without removing the sample. This is done by adding silica gel to the injection column followed by the sample that has been dissolved in a soluble solvent. Some of the solvent is allowed to evaporate and the injection column is placed on a standard rotary evaporator with the EZ-Pass adapter and the remaining solvent removed with no loss of silica gel into the evaporator. Additional drying can be done on a vacuum line if desired. The dry pre-absorbed sample in the injection column in placed on top of the main column and separated.