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Competitor Comparison - Columns

Comparison of RT Scientific 43g Glass Column with Competitor's 40g and 35g Plastic Cartridge

Since our columns are packed even and tight, they give better separations than hand packed columns or plastic cartridges. To demonstrate the performance of our columns we have compared ours with plastic cartridges.

 We separated a sample on each column/cartridge being tested. We used our PurChrom 35-G system, keeping the method of injection and gradient the same. The results are given below:  

Sample: 2-Chloro-5-nitropyridine (2 g), 2,3-Dimethoxybenzaldehyde (9 g) and 3,4-dimethoxy- benzaldehyde (12.5 g) was dissolved in 8 ml of ethyl acetate and 38 ml of hexanes. This gave a standard solution of 70 ml.

Conditioned the column with 70 to 300 ml of 3% ethyl acetate/97% hexanes. Inject sample as indicated in the table with a 5 ml syringe. Run for 15 minutes in 3% ethyl acetate/97% hexanes and start linear gradient at 3% ethyl acetate/97% hexanes to 18% ethyl acetate/82% hexanes over a period of 68 minutes. Fractions of 15 ml were collected after the 1st fraction as indicated in the table. Run time and run volumes were determined from injection to when the last fraction of compound 3 came off (based on TLC). TLC was performed in 20% ethyl acetate/ 80% hexanes. Rf values were 0.57, 0.47 and 0.25 for the respective compounds above.

Column Sample Load Flow Rate in ml/min. Pressure Run Time 1st Fraction Volume
RTSI 43g 1175 mg (3.5 ml) 15.8 18 psi 48 min. 94 ml
Competitor #1 40g 1091 mg (3.25 ml) 17.5 2 psi 67 min. 85 ml
Competitor #2 35 g 956 mg (2.85 ml) 17.2 5 psi 49 min. 87  ml
           
  1st Compound
Total Fractions		 Impure (Overlap) Fractions 2nd Compound
Total Fractions		 3rd Compound
Total Fractions		 Total Run Volume
RTSI 43g 8 1 9 11 754 ml
Competitor #1 40g 8 3 12 33 1195 ml
Competitor #2 35 g 7 5 12 23 837 ml
           
  1st Compound Pure 1st and 2nd Compound Overlap 2nd Compound Pure 3rd Compound Pure Total Recovery
RTSI 43g 90 mg [100 mg]* 39 mg (3.3%)** 398 mg [450 mg] 600 mg [625 mg] 1127 mg (96%)
Competitor #1 40g 73 mg [93 mg] 133 mg (12.2%)** 291 mg [418 mg] 448 mg [580 mg] 945 mg (87%)
Competitor #2 35 g 28 mg [81 mg] 186 mg (19.5%)** 226 mg [366 mg] 299 mg [509 mg] 739 mg (77%)

* Contained in the bracket [] is the theoretical amount that should be recovered.
** Percent of impure fractions.


RT Scientific 43 g
 Column

Competitor #1
 40 g Cartridge

Competitor #2
 35 g Cartridge