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Choosing Column Sizes
We offer columns from 6 grams to 1.3 Kg to accomplish most
separations. Using the chart below will help in selecting the right column for
your separation using normal phase silica.
Column Selection
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Sample Purification
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Column Size
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DRf 0.1
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DRf 0.3
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6 g (0.8cm x 25 cm)
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150 mg
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300 mg
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14 g (1.1 cm x 30 cm)
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380 mg
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900 mg
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16 g (2.2 cm x 13 cm)
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435 mg
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1 g
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35 g (25 cm x 15 cm)
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950 mg
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2.3 g
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43 g (22 cm x 30 cm)
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1.1 g
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2.9 g
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80 g (4.0 cm x 17 cm)
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2.1 g
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5.4 g
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115 g (3.3 cm x 30 cm)
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3 g
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7.5 g
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170 g (4.0 cm x 35 cm)
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4.6 g
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11.5 g
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370 g (5.1 cm x 45 cm)
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10 g
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25 g
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850 g (6.7 cm x 58 cm)
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22 g
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57 g
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1.3 Kg (7.9 cm x 62 cm)
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33 g
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88 g
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Note: The above numbers
may vary depending on the solvent conditions used.
DRf is the difference in Rf valves with
the top spot moving 50% up the plate. Example: Top spot Rf = 0.5 and
the bottom spot Rf = 0.4, DRf =
0.5-0.4 = 0.1
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Flow rates of
columns with 25% Ethyl acetate/75% Hexane
Our packed ACE Glass Inc.,
Michel-Miller column with PTFE fittings results in a product that is inert to
most chromatography conditions. Our columns will not swell or leak as plastic
cartridges do. The packing process results in columns that are packed tight for
repeated use or preconditioning and use later. However, we have priced our
columns to that of one-time use columns (see the columns section for pricing).
Below is a table of flow rates of our normal phase silica packed columns:
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Column
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10 psi
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15 psi
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20 psi
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25 psi
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30 psi
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35 psi
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0.8 cm x 25 cm, 6 g
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2.6 mL
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3.8 mL
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4.9 mL
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6.0 mL
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7.2 ml
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8.2 mL
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1.1 cm x 30 cm, 14 g
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4.4 mL
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6.4 mL
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8.0 mL
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10.2 mL
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12.2 mL
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14.1 mL
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2.2 cm x 13 cm, 16 g
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12.8 mL
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18.0 mL
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23.0 mL
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27.0 mL
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32.0 mL
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36.0 mL
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2.7 cm x 15 cm, 35 g
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15.0 mL
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22.5 mL
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28.0 mL
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34.0 mL
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39.0 mL
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43.0 mL
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2.2 cm x 30 cm, 43 g
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7.5 mL
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11.5 mL
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14.5 mL
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18.0 mL
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20.7 mL
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24.8 mL
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4.0 cm x 16 cm, 80 g
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20.6 mL
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26.7 mL
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34.5 mL
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42.5 mL
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48.6 mL
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54.5 mL
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3.3 cm x 30 cm, 115 g
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14.5 mL
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19.3 mL
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24.0 mL
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30.0 mL
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35.5 mL
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39.0 mL
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4.0 cm x 35 cm, 170 g
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12.9 mL
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21.8 mL
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30.0 mL
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36.0 mL
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44.0 mL
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50.0 mL
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5.1 cm x 45 cm, 370 g
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20.5 mL
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30.0 mL
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38.0 mL
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48.5 mL
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57.0 mL
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65.0 mL
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6.7 cm x 58 cm, 880 g
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20.5 mL
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30.0 mL
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37.5 mL
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46.5 mL
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51.0 mL
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57.5 mL
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7.9 cm x 62 cm, 1.3 Kg
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14.0 mL
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17.5 mL
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23.5 mL
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29.0 mL
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34.0 mL
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38.0 mL
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The flow rates of the 880 g and 1.3 Kg at 50 psi are
73.0 mL and 53.0 mL respectively.
Dead Volume (Air space) of columns is approximately
1.25 ml for every 1 gram of silica gel.
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